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Standard curves of the three optimized Eimeria DNA extraction kits. Spiking samples were prepared by stepwise dilution from 10 5 to 10 3 concentrations. Ct values are plotted against the log of the starting quantity, with corresponding regression equations and coefficients of determination (R²) shown for each kit.

Journal: Poultry Science

Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR

doi: 10.1016/j.psj.2026.106971

Figure Lengend Snippet: Standard curves of the three optimized Eimeria DNA extraction kits. Spiking samples were prepared by stepwise dilution from 10 5 to 10 3 concentrations. Ct values are plotted against the log of the starting quantity, with corresponding regression equations and coefficients of determination (R²) shown for each kit.

Article Snippet: DNA was extracted from spiked samples with the vaccine EVALON® using three manual extraction systems and six automated extraction systems according to the manufactures’ instructions: the manual spin-column-based (1) DNeasy Blood & Tissue Kit (QIAGEN), (2) QIAamp Fast DNA Stool Mini Kit (QIAGEN), and (3) PURE TM Soil · Fecal gDNA Extraction Kit (Infusion Tech, Anyang, Korea), and the automated magnetic bead-based nucleic acid extraction system (4) Sporozoa kit using the AP16 nucleic acid purification system (APBio, Namyangju, Korea), (5) TANBead Universal Pathogen Extraction Kit using the Maelstrom 4810 automated nucleic acid extractor (Taiwan Advanced Nanotech, Taoyuan, Taiwan), (6) TANBead OpiPure Viral Auto Plate Kit using the Maelstrom 4810 automated nucleic acid extractor (Taiwan Advanced Nanotech), (7) TANBead Tissue Total DNA Extraction Kit using the Maelstrom 4810 automated nucleic acid extractor (Taiwan Advanced Nanotech), (8) MaxwellRCS PureFood GMO and Authentication Kit using the Maxwell 48 RSC extraction system (Promega, Madison, WI, USA), and (9) Maxwell RSC simply RNA Tissue Kit using the Maxwell 48 RSC extraction system (Promega).

Techniques: DNA Extraction